Associate Specialist (Protein Production) - Antibiome Center

Associate (Protein Production)

Antibiome Center, Department of Pharmaceutical Chemistry UCSF

The laboratory of Jim Wells and the Antibiome Center is seeking a highly motivated candidate to join our quick paced and multi-talented team. The successful candidate will be responsible for molecular biology, protein/antibody expression and purification, and protein/antibody validation. The candidate will have opportunities to learn state-of-the-art techniques. Our laboratory is highly collaborative and is engaged in numerous academic and industrial collaborative projects.

The Antibiome Center is part of the Recombinant Antibody Network (RAN), a collaborative consortium consisting of laboratories at the University of California San Francisco (Dr. Jim Wells), University of Chicago (Dr. Tony Kossiakoff), and the University of Toronto (Dr. Sachdev Sidhu). We are committed to generate recombinant antibodies at the surface proteome-wide scale using high throughput phage display approaches. Our long history of protein engineering expertise also enables the development of bio-therapeutic antibodies in the immuno-oncology field.

Primary responsibilities

• Cloning recombinant protein and antibody construct using a variety of molecular biology techniques including Polymerase Chain Reaction, Gibson assembly, DNA purification, and transformation.

• Protein expression from bacterial and mammalian sources.

• Purification of proteins using chromatography.

• Characterization of proteins by variety of techniques including SDS-PAGE, ELISA, SEC, and Western Blotting.

Required Qualifications

• MS or PhD in relevant life sciences field or at least five years of research experience for Associate Specialist rank.

Preferred Qualifications

• Familiar with mammalian tissue culture techniques including transfections, cell line maintenance, recombinant protein expression.

• Proficiency with advance biophysical and structure characterization of proteins and antibodies.

• Experience with any automation and robotics platform.

Please apply online at https://aprecruit.ucsf.edu/JPF04103 with a CV, cover letter, and the contact information of 3 references.