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Job Summary

The Aparicio lab is developing new single cell genome and transcriptome methods for tracking clonal trajectories at single cell level in patient tissues and to gain a better understanding of tumor microenvironment. Researchers uses the latest Single Cell Genomics technologies to rapidly identify genes that are involved in the development of cancer. This position will be working on the Single Cell Genomic research projects currently on-going in the research lab, providing technical guidance, expertise, and support to other internal/external researchers on Single Cell Genomics, in addition, helping with continual development and on-going production at 2 separate research facilities located in Vancouver, B.C.

We are seeking a highly-motivated and highly skilled individual with expertise or experience in single cell genomics, robotics, next-generation sequencing and a strong background in molecular biology. The successful applicant will work full-time under the direction and supervision of both Dr. Samuel Aparicio, Principal Investigator, and the Laboratory Research and Operations manager. Individual should possess a strong molecular biology background; experience with liquid handling robotics is considered an asset. Experience with bioinformatics approaches to biological data and familiarity with handling of genomics and next-generation sequencing data will be an advantage. This position will provide the candidate with a high level of exposure to single cell genomics and translational research, as well as, providing opportunities to work collaboratively with industry partners and other researchers globally; position start date is flexible.

Organizational Status

Aparicio Lab is located in the department of Molecular Oncology at BC Cancer Research Institute in Vancouver, British Columbia, Canada. The candidate will report directly to Dr. Samuel Aparicio, or Laboratory Research and Operations Manager, in addition, the applicant may assist in overseeing technical work of students and trainees.

Work Performed

  • Plans and performs experiments utilizing established complex procedures and/or techniques for investigating single cell genomics, involving the use of liquid handling robotics and 10x genomics platforms
  • Visualize and analyze cells using microscopy and flowcytometry.
  • Generation, preparation and submission of next-generation sequencing libraries.
  • Using a LMIS system to track and record sample data
  • Provide technical expertise and support in molecular biology, protein biochemistry, tissue culture and virology
  • Develop and implement standard operating protocols (SOPs).
  • Maintains laboratory records and experimental data.
  • Assist in general lab maintenance such as reagent preparation, receiving and inventory of lab supplies.
  • Conducts complex data analysis and interpretation of data resulting from high throughput experiments.
  • Presents data at scientific meetings, writes reports and contributes to writing ethics and grant applications.
  • Carries out any other related duties as assigned or required in keeping with the qualifications and requirements of this technician level.

Consequence of Error/Judgement

The work is of a complex nature and required high level of training, tasks are assigned and the incumbent exercises a considerable amount of responsibility, initiative and judgment in determining own work schedule to achieve those tasks. Errors may result in the permanent loss of irreplaceable tissue samples, loss of thousands of dollars of material and/or damage to equipment. Incorrect decisions or actions may damage the reputation of the laboratory, lead to loss of credibility in this field, and be financially costly. Noncompliance with biosafety and chemical safety regulations may also result in closure of facility or loss of grant funding.

Supervision Received
The successful applicant will work under minimum supervision. From time to time, Principal Investigator or Laboratory Research and Operations Manager may provide specific instructions on unusual problems, or new project outlines. This position will report to the Principal Investigator or Laboratory Research and Operations Manager.

Supervision Given
Depending on experience and expertise, the successful applicant maybe be required to assist in training and supervision of graduate students/trainees, junior staff, or external collaborators; including providing instruction on use of lab equipment and procedures, and will help troubleshoot experiments in their area of expertise.

Minimum Qualifications

  • Completion of a university degree in a relevant discipline or technical program and a minimum four years of related experience or an equivalent combination of education and experience. Some positions may require a graduate degree.
  • A MSc in Biological Sciences (Molecular Biology or other relevant field) or BSc with 4-5 years of related experience in academic research or biotech industry settings, preference will be given to applicants with work experience in single cell genomics biology or molecular biology.

Preferred Qualifications

  • A working knowledge and understanding of molecular biology and biochemistry techniques such as next-generation sequencing, PCR, DNA/RNA purification and cloning techniques is essential; prior working experience in next-generation sequencing and library construction is considered a great asset.
  • Experience with cell culture and aseptic technique.
  • Working hands-on experience in tissue handling and tissue dissociation methods would be an asset.
  • Ability to perform and troubleshoot a wide variety of molecular and cellular biology techniques including the following: RNA / DNA isolation, quantitative PCR, transfection, flow cytometry, immunohistochemistry, ELISA, protein isolation, and western blotting.
  • Preference will be given to applicants with liquid handling robotics, single cell genomics, next-generation sequencing, and 10x Genomics experience.
  • Experience in immunocytochemistry and basic data analysis in R or similar statistics package would be an asset.
  • Demonstrated understanding of experimental design and assay optimization.
  • Strong interpersonal skills, communication skills (orally and written) and the ability to interact positively and productively with other team members are essential.
  • Excellent documentation and organizational skills.
  • Able to multi-task, prioritize and handle multi-project assignments concurrently.

The successful candidate must be well-organized, conscientious, understand the importance of detail, and be able to multi-task and prioritize duties effectively.

Application

Please apply directly by clicking the green "Apply Externally" button.

Compensation Range

$4,558.75 - $5,354.67 CAD Monthly

Job End Date

Dec 5, 2022

The anticipated start date for this position is December 6, 2021. The term is for one year with the possibility of extension.

In your application please include (1) a cover letter, and (2) a CV or resume.

All qualified candidates are encouraged to apply; however, Canadians and permanent residents will be given priority.

This position is located within a health-care facility. Therefore, this positions requires successful verification of full vaccination against Covid-19 provided prior to the start date, as required by the provincial health mandate.

This posting is for the UBC Vancouver campus in British Columbia, Canada.

Please refer to reference number HS-55583 during correspondence about this position.

Equity and diversity are essential to academic excellence. An open and diverse community fosters the inclusion of voices that have been underrepresented or discouraged. We encourage applications from members of groups that have been marginalized on any grounds enumerated under the B.C. Human Rights Code, including sex, sexual orientation, gender identity or expression, racialization, disability, political belief, religion, marital or family status, age, and/or status as a First Nation, Metis, Inuit, or Indigenous person.

All qualified candidates are encouraged to apply; however Canadians and permanent residents will be given priority.

About UBC's Faculty of Medicine

Our Vision: To Transform Health for Everyone.

Ranked among the world's top medical schools with the fifth-largest MD enrollment in North America, the UBC Faculty of Medicine is a leader in both the science and the practice of medicine. Across British Columbia, more than 11,000 faculty and staff are training the next generation of doctors, health care professionals and scientists, making remarkable discoveries, and helping to create the pathways to better health for our communities at home and around the world.

The Faculty of Medicine - comprised of approximately 2,200 administrative support, technical/research and management and professional staff, as well approximately 650 full-time academic and more than 9,000 clinical faculty members - is composed of 19 academic basic science and/or clinical departments, three schools, and 24 research centres and institutes. Together with its University and Health Authority partners, the Faculty delivers innovative programs and conducts research in the areas of health and life sciences. Faculty, staff and trainees are located at university campuses, clinical academic campuses in hospital settings and other regionally based centres across the province.